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KMID : 1145120190170010107
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Asian Journal of Beauty and Cosmetology
2019 Volume.17 No. 1 p.107 ~ p.115
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Anti-inflammatory Effect of Brazilin in RAW264.7 Cells
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Shim Joong-Hyun
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Abstract
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Purpose: This study aimed to determine the skin anti-inflammatory effect of brazilin on RAW264.7 cells.
Method: To determine anti-inflammatory effects of brazilin on RAW264.7 cells, we measured cell viability, gene expressions, and prostaglandin E2 (PGE2)/nitric oxide (NO) production.
Results: Cells were treated with brazilin to confirm cytotoxicity; no toxicity was observed when cells were treated at a concentration of ≤1 μg/mL. In RAW264.7 cells, the number of intracellular granule were increased after lipopolysaccharide (LPS) treatment; however, brazilin treatment decreased intracellular granules similar to that in the control group. In this research, we elucidated the anti-inflammatory effects of brazilin on the mRNA expression of interleukin 1 alpha (IL1α ), interleukin 1 beta (IL1β ), interleukin 6 (IL6 ), and tumor necrosis factor alpha (TNFα ) and PGE2/NO production. Real-time reverse transcription polymerase chain reaction (real-time RT-PCR) showed that brazilin administration increased the mRNA levels of IL1α /IL1β /IL6 /TNFα genes, and analysis of PGE2/NO also revealed that brazilin induces anti-inflammatory effects.
Conclusion: We identified the anti-inflammatory effect of brazilin, and found that brazilin can be a potent cosmetic ingredient for anti-inflammatory agent for atopic dermatitis. Based on this result, we believe that brazilin has the potential to be used in not only cosmetics, but also medicine or healthcare food.
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KEYWORD
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Brazilin, Anti-inflammatory, Nitric oxide, PGE2, TNFα
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